EFFECT OF SELENIUM AND /OR VITAMIN E ON BOVINE HERPES TYPE 1 INFECTION IN VITRO CULTURED CELLS

Umima M Mansour ,Hala A Salem

doi:10.21608/avmj.2015.170217

Umima M Mansour , Hala A Salem

Open Access

https://doi.org/10.21608/avmj.2015.170217

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Abstract

Bovine herpes virus type 1 (BHV-1) still the causes of great economic lossesin the livestock industry and trade because there are no available drug that proved to be fully effective against it. In this study, the antioxidant of selenium and/ or the vitamin E were evaluated as a natural anti-viral drugs. The antiviral activities against Bovine Herpesvirus type 1 (BHV-1) were evaluated by the reduction of the viral cytopathic effect 1nMadin-Darby Bovine Kidney cell line (MDBK) with, GSH (glutathione reduced) and MAD (malondialdehyde) assays. Selenium in concentrations 0.01 and 0.1 µM and / or Vit. E in concentrations 50 and 100 µM with two concentrations were added at different stages of the viral infection (pre and post treatment). This study concluded that the higher concentration of Selenium and Vit. E (0.1 µM+100µM respectively) reduced the Cytopathic Effect (CPE) of BHV-1 1n 50% and increased GSH level as well as decreased MDA level, in pre infection treatment assay.

Highlights

Bovine herpesvirus-1 (BHV-1), called infectious bovine rhinotracheitis/ infectious pustular vulvovaginitis virus, is a member of the subfamily Alphaherpesvirinae and is one of the most common viral pathogens found in bovine semen (Afshar and Eaglesome., 1990)
Table (2) shows effect of selenium or / and vitamin E treatments applied with different concentrations in pre-infection protocol on Cytopathic Effect (CPE)%, GSH and MDA levelsinMDBK cellsby BHV-1
Our results showed that the lipid- peroxidation in Madin Darby Bovine Kidneycells (MDBK) cells virus control cells, expressed as the level of MDA (5.27 ±0.15 nmol/L), was significantly higher (p>0.05) in comparison with cell control (0.15±0.03nmol/L), as well as in comparison with the levels of MDA during selenium treatment alone

Summary

INTRODUCTION

Bovine herpesvirus-1 (BHV-1), called infectious bovine rhinotracheitis/ infectious pustular vulvovaginitis virus, is a member of the subfamily Alphaherpesvirinae and is one of the most common viral pathogens found in bovine semen (Afshar and Eaglesome., 1990). Selenium and vitamin E deficiency increase viral disease progression with Coxsackie virus in mice (Beck et al, 1994 and Beck et al, 1995). A similar finding was observed when an attenuated strain of influenza A rapidly gained virulence upon passage through selenium deficient mice (Nelson et al, 2001) These results suggest dietary effects on oxidative stress may influence viral disease progression. Preinfection treatment assay: Confluent monolayer cultures of MDBK cells were grown in 24 wellplates, treated with different concentration of selenium and vitamin E, incubated for 24 h at 37°C and infected with BHV-1. - Postinfection treatment assay: Confluent monolayer cultures of MDBK cells were grown in 24 wellplates, inoculated by BHV-1, incubated for 24 h at 37°C and treated with different concentration of selenium and vitamin E. The statistical significance of observed differences between means was determined using ANOVA for comparing means of different samples and was defined as p > 0.05 according to Snedecor and Cochran (1987)

RESULTS

DISCUSSION

CONCLUSION