Abstract
Objective To analyze the effect of the fracture healing by using selective sensory radicotomy before and after the ganglion. Methods From September, 2014 to August, 2015 , 120 rats were averagely divided into 3 groups, which was the sham operate group (SO) , the anterior injury of ganglion rhizotomy group (AGR) and the posterior injury of ganglion rhizotomy group (PGR) . Tibial transverse fracture was performed on right or left randomly and the fracture was fixed with intramedullary. In coordination with the fractured- side, the selective radicotomy from L4 to L6 was made. The mRNA level of collagen-I and collagen-II in bony callus was quantified at 3, 7 days and 2 weeks after the operation by RT-PCR (n=5) . The histological examination, X-ray, biomechanical testing were performed at 4 and 8 weeks. The quantitative data of iconography and biomechanics was acquired by using the relative ration which resulted from fractured-side data dividing the unfractured-side one for every pair tibia in same rat. Results At 3 days after surgery, the mRNA of collagen-I were 81.3±11.1, 37.5±8.2 and 24.7±9.2, the mRNA of collagen-Ⅱwere 2.4±0.5, 2.1±1.0 and 2.9±1.2 in SO, AGR and PGR group respectively; At 7 days after surgery, the mRNA of collagen-I were 17.9±5.8, 7.2±1.8 and 6.7±2.4, the mRNA of collagen-Ⅱwere 12.5±3.4, 2.8±1.3 and 1.2±0.4 in SO, AGR and PGR group respectively. The SO group had significantly upregulation of collagen-I and collagen-II mRNA compared with the AGR and PGR groups at 3 days and 7 days respectively after surgery (P<0.05) . There was a significant difference of the collagen-II between AGR group (2.8±1.3) and PGR group (1.2±0.4) at 7 days after injury (P<0.05) . The fracture bony callus in PGR group was a significantly larger and less ossified than that in the AGR group. The mechanical load of fracture in SO group was larger than that of the other 2 groups (P<0.05) . Conclusion Sensory denervation negatively affects fracture-healing. The ganglion of sensory nerve plays an important role in bone fracture healing and normal innervation is essential for the fracture repair. Key words: Ganglion; Sensory nerve; Radicotomy; Fracture healing
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
