Effect of Selective Portal Vein Occlusion on Hepatic Reduced Glutathione and Lipid Peroxidation Expression in Regeneration and Atrophy of Hepatic Lobes

Abstract

This study aimed to clarify the mechanisms by which selective portal vein occlusion can result in hypertrophy of the remnant liver and atrophy of the liver to be resected. The association between hepatic reduced glutathione (GSH) and malondialdehyde (MDA) in lobes supplied by branches of the portal vein that had been occluded (LPVO) or not occluded (LPVNO) were evaluated in rats. Changes in liver function in both groups were determined before and after portal vein occlusion (PVO) . Transaminase showed significantly higher levels in the PVO group than in the sham operation group on days 1 and 3, and the levels of prothrombin activity in the PVO group were significantly lower than in the sham group on day 1. GSH in the LPVNO increased significantly during the first 3 days in comparison to the sham group, and reached a peak on the 3rd day. In the LPVO, the levels of GSH decreased gradually relative to the sham group on days 3, 5, and 7 MDA levels in both LPVNO and LPVO were significantly higher on the first day than in the sham group. From the third day. MDA levels in the LPVNO were less than the lowest level in the sham group, while those in the LPVO continued to increase linearly throughout the observation period. The correlations between GSH and MDA levels in the LPVO were negatively significant. The regeneration in LPVNO was preceded by a temporary increase in MDA and a subsequent increase in GSH production, whereas LPVO showed atrophic changes with a continuous decrease in GSH and an increase in MDA.