Abstract

Helminthosporium oryzae, a fungal pathogen causing Brown Spot of Rice is one of the disastrous biotic factors responsible for 16–43% yield in rice. To control in vitro fungal growth of this pathogen, four fungicides viz. Mencozeb, Thiophanate Methyl, Iprovalicarb + Propineb and Propineb were used with four different concentrations: 50, 100, 150 and 200 ppm. Plant extracts of five species as Azadirachta indica, Calotropisprocera, Allium sativum, Datura stramonium and Zingiber officinale were also tested with three doses (5, 10 and 15 ml). While, 24 isolates of Bacterial isolates were investigated for their antagonistic effect. The in vitro results of the four fungicides contributed that Mancozeb and Thiophanate Methyl inhibited the colony growth at the higher doses (150 and 200 ppm) with no linear colony growth. However, Propineb was found moderately effective at (5.00 mm) and Iprovalicarb + Propineb was less effective (7.25 mm). Similarly, the in-vitro efficacy of the five plant extracts against H. oryzae at different doses revealed that Zingiber officinale and Allium sativum were more effective at high doses (00.00 mm, 2. mm). Also, Datura stramonium (4.62 mm) Azadirachta indica (18.00 mm) and Calotropisprocera (12.00 mm) performed better as compared to the control (40.00 mm). The results revealed that out of 24 isolates, 4 isolates of bacteria Agrobacterium spp, Xanthomonas, Erwinia, Streptomyces has inhibited the linear colony growth of H. oryzae up to 1-2 mm. This study can be useful for controlling the fungal disease as the two fungicides Mancozeb and Thiophanate Methyl were more effective and the Zingiber officinale and Allium sativum plant extracts were highly effective against the brown spot of rice. Biocontrol also reduced leaner colony Helminthosporium oryzae.

Talk to us

Join us for a 30 min session where you can share your feedback and ask us any queries you have

Schedule a call

Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.