Effect of season on germinal vesicle stage, quality, and subsequent in vitro developmental competence in bovine cumulus-oocyte complexes.

Abstract

Although the reproductive performance of grazing cattle is lower in summer compared to winter, the effect of season on oocyte developmental competence has not been thoroughly examined. We measured the effect of season on oocyte chromatin compaction, cumulus cell quality, and embryonic development after in vitro fertilization. Cumulus oocytes-complexes (COCs) were collected from abattoir cows' ovaries during the winter and summer months. First, we evaluated the degree of chromatin compaction in germinal vesicle (GV) oocytes (GV1 through GV3), which is associated with different degrees of developmental competence. Then, we determined the apoptotic index in cumulus cells from immature and in vitro matured COCs. Finally, in vitro matured oocytes were fertilized to determine blastocyst rate and embryo quality. During the summer months, we observed a significantly lower proportion of oocytes reaching the GV3 stage and higher levels of DNA fragmentation in cumulus cell. As a result, blastocyst yield and quality were reduced during the summer months. In conclusion, summer negatively affected oocyte GV stage progression, cumulus cell quality, and embryo development. Increased cumulus cell DNA fragmentation during summer, may partially explain the reduced oocyte maturation capacity, considering the relevance of cumulus-oocyte communication during this stage.