Abstract
BackgroundSaururus chinensis leaves have been used as traditional medicine in Korea for pain, intoxication, edema, and furuncle. According to previous reports, these leaves exert renoprotective, neuroprotective, and antioxidant effects by attenuating inflammatory responses. However, the beneficial effect of Saururus chinensis leaves on arthritis has not been elucidated. Thus, we evaluated the water extract of Saururus chinensis leaves (SHW) using type II collagen-induced arthritis (CIA) mice models.MethodsQuantitative analysis of major components from SHW was performed by HPLC. Arthritis was induced by injection of type II collagen. Each group was orally administered SHW (100 mg/kg and 500 mg/kg). Methotrexate (MTX) was used as a positive control. Serum levels of interleukin-6, TNF-alpha, and type II collagen IgG in the animal models were measured using ELISA. Histological features were observed by H&E staining.ResultsQuantitative analysis of SHW showed the contents as 56.4 ± 0.52 mg/g of miquelianin, 7.75 ± 0.08 mg/g of quercetin 3-O-(2”-O-β -glucopyranosyl)-α-rhamnopyranoside, and 3.17 ± 0.02 mg/g of quercitrin. Treatment with 500 mg/kg SHW decreased the serum level of Interleukin-6 (IL-6), TNF-alpha, and collagen IgG in the CIA model. Moreover, SHW treatment diminished the swelling of hind limbs and monocyte infiltration in blood vessels in CIA animal models. The results indicate that SHW could decrease CIA-induced arthritis in vivo.ConclusionsThe results indicate that SHW could be used to improving arthritis by reducing inflammatory factors (IL-6 and TNF-alpha). However, further experiments are required to determine how SHW influences signal transduction in animal models.
Highlights
Saururus chinensis leaves have been used as traditional medicine in Korea for pain, intoxication, edema, and furuncle
The water extract of Saururus chinensis leaves (SHW) administration decreased the serum level of IL-6 and TNF-alpha and did not influence toxicity markers of the liver and kidneys in collagen-induced arthritis (CIA) animal models To confirm the toxicity of SHW on the liver and kidneys, we evaluated the commonly used toxicity markers including Blood urine nitrogen (BUN), Cre, Aspartate serum transferase (AST), and Alanine amino transferase (ALT)
This study demonstrated the anti-inflammatory effect of SHW administration in CIA animal models through improved inflammatory responses such as elevated IL-6, TNF-alpha, and type II collagen IgG in serum, as well as swelling of the hind limbs
Summary
Saururus chinensis leaves have been used as traditional medicine in Korea for pain, intoxication, edema, and furuncle. The extract of Saururus chinensis (leaves, stems and flowers) shows renoprotective and antioxidant effects in rats fed a high-fructose diet [2], and inflammation-mediated neurotoxicity was found to be attenuated by treatment with the Rheumatoid arthritis (RA) is one of the most common autoimmune diseases, affecting about 2% of the world population [10]. It is characterized by destruction of the. Most drugs used for the treatment of RA have anti-inflammatory and anti-oxidative stress effects [14]
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