Abstract
BackgroundSalvia leriifolia have been shown to decrease ischemia-reperfusion (I/R) injury in brain tissues. In this study, the effects of S. leriifolia aqueous and ethanolic extracts were evaluated on an animal model of I/R injury in the rat hind limb.MethodsIschemia was induced using free-flap surgery in skeletal muscle. The aqueous and ethanolic extracts of S. leriifolia (100, 200 and 400 mg/kg) root and normal saline (10 ml/kg) were administered intraperitoneally 1 h prior reperfusion. During preischemia, ischemia and reperfusion conditions the electromyographic (EMG) potentials in the muscles were recorded. The markers of oxidative stress including thiobarbituric acid reactive substances (TBARS), total sulfhydryl (SH) groups and antioxidant capacity of muscle (using FRAP assay) were measured.ResultsIn peripheral ischemia, the average peak-to-peak amplitude during ischemic-reperfusion was found to be significantly larger in extracts groups in comparison with control group. Following extracts administration, the total SH contents and antioxidant capacity were elevated in muscle flap. The MDA level was also declined significantly in test groups.ConclusionIt is concluded that S. leriifolia root extracts have some protective effects on different markers of oxidative damage in muscle tissue injury caused by lower limb ischemia-reperfusion.
Highlights
Salvia leriifolia have been shown to decrease ischemia-reperfusion (I/R) injury in brain tissues
EdFfaifgteiucotrneofof2Sllaolwviainlgermiifuoslicaleroisocthaeqmuieaoruesperxftursaicotnoinjulipryid peroxiEffect of Salvia leriifolia root aqueous extract on lipid peroxidation following muscle ischemia reperfusion injury.MDA levels were measured in 10% homogenates of muscle samples from rats subjected to 120 min of ischemia and 60 min of reperfusion
Ferric Reducing/Antioxidant Power (FRAP) values were measured in 10% homogenate samples from rats subjected to 120 min of ischemia and 60 min of reperfusion
Summary
Salvia leriifolia have been shown to decrease ischemia-reperfusion (I/R) injury in brain tissues. The effects of S. leriifolia aqueous and ethanolic extracts were evaluated on an animal model of I/R injury in the rat hind limb. The oxidant injury can potentially occur during ischemia and reperfusion due to an excess production of oxygen free radicals, a decrease in antioxidant defenses, or both. Because antioxidants function by removing the toxic oxygen metabolites, they are generally highly effective in reducing ischemia-reperfusion injury [2]. The plants of the genus Salvia, which consist about 900 species [5] are generally known for their multiple pharmacological effects such as analgesic and anti-inflammatory [6], antioxidant [7], hepatoprotective [8] and hypoglycemic activities [9]. The different pharmacological activities of this plant such as the attenuation of morphine dependence [11], hypoglycemic [12], analgesic and anti-inflammatory [13,14], anticonvulsant [15], antiulcer effects [16] and antibacterial activities [17] were evaluated in our laboratory
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