Effect of salt and metal accumulation on performance of membrane distillation system and microbial community succession in membrane biofilms

Abstract

Membrane distillation (MD) works as a potential technology for the “zero liquid discharge” water treatment owing to its high concentration brine tolerance. The continuous accumulation of salts and metals in the MD system during the “zero liquid discharge” water treatment inevitably posed remarkable impacts on the biofilm formation as well as the MD performance. Hence, the biofouling mechanism of MD was deeply researched in this study with an emphasis on the roles of salt-stress (NaCl) and metal-stress (Zn and Fe) in biofilm development. The membrane flux decline of MD was effectively mitigated by the appearance of NaCl and ZnO, while that was significantly aggravated under the metal-stress of Fe. Considering the serious membrane scaling caused by NaCl crystals, a sharp flux decline was seen for the NaCl group during the later stage of MD operation. Basing on the 16S rDNA and 16S rRNA analysis, heat-stress, salt-stress, and metal-stress all posed certain impacts on the biofouling development in the MD system, and a more remarkable influence was observed for metal-stress. Under the salt-stress from NaCl, a thin biofilm containing high biovolume of dead cells finally formed, in which the bacterial community mainly consisted of halotolerant and thermophile species. Owing to the Zn2+-stress and oxidation-stress mechanisms of ZnO, the bacteria in the MD system were largely dead and live bacterial community in biofilms was dominated by some gram-negative species. Under the metal-stress from Fe, a rather thick biofilm containing higher biovolume of live cells clearly developed, in which the prevailing species could secret large amounts of EPS and accumulate metabolites around cells as biological surfactants, inducing aggravated membrane biofouling and high risk of membrane wetting.