Effect of Salmonella typhimurium A1-R on a pancreatic cancer patient-derived orthotopic xenograft (PDOX) in nude mice.

Abstract

e15218 Background: Tumor-targeting Salmonella typhimurium A1-R (A1-R) has shown great promise in cell lines of pancreatic and other cancer in nude mouse models. The present result demonstrate efficacy of Salmonella typhimurium A1-R on a more clinically relevant pancreatic cancer patient-derived orthotopic xenografts (PDOX). Methods: The PDOX model was originally established from a pancreatic cancer patient in SCID-NOD mice. The pancreatic cancer PDOX was subsequently transplanted by surgical orthotopic implantation (SOI) in transgenic nude red fluorescent protein (RFP) mice in order that the PDOX stably acquired red fluorescent protein (RFP)-expressing stroma for the purposes of imaging the tumor after passage to non-transgenic nude mice in order to visualize tumor growth and drug efficacy. The nude mice with human pancreatic PDOX were treated with A1-R or standard chemotherapy, including gemcitabine (GEM), which is first-line therapy for pancreatic cancer, for comparison of efficacy. Results: A1-R treatm...