Abstract
The present study analysed the effect of salinity stress on survival, biochemical constituents such as soluble protein, amino acid, trehalose and fatty acid, as well as real time expression of the Artemia heat-shock protein 22 (ArHsp 22) gene in Artemia franciscana. Results of the study revealed that Artemia can withstand sudden salinity increase up to 200 ppt without any mortality up to 6 h. Significantly higher mortality percentage was recorded at 24 h of incubation at 200 ppt. Short-term exposure to hypersaline conditions significantly reduced the protein content in Artemia while the protein biosynthesis enhanced after 24 h of incubation at 150 ppt salinity. Long term exposure to 200 ppt salinity did not show any further increase in amino acid content as observed at 100 and 150 ppt conditions, indicating reduced rate of amino acid metabolism and stress. Salt stress induced the synthesis of total polyunsaturated fatty acids (PUFA) in Artemia especially the 22:6n-3 and 20:5n-3 after 24 h of incubation at 150 ppt . Exposure to higher salinity induced trehalose production which indicated its vital role in combating salt stress in Artemia. The present study also indicated that short-term salt stress can significantly enhance the ArHsp22 gene expression in Artemia adults.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
