Abstract
1. 1. During the same culture period, Oreochromis niloticus raised in sea-water grew almost 60% slower than that raised in fresh water. 2. 2. Blood osmolarity of the sea-water specimen was 380–390 mOsm and that of the fresh water specimen was 280–300 mOsm. 3. 3. The potency of proteases from sea-water specimens were similar to those from fresh water specimens when assayed in high salt medium (30%. NaCl). However, it was decreased significantly when assayed in normal Tris-buffer medium. 4. 4. The activity of the enzymes from fresh water specimens did not change significantly in a salinity range from less than 0.1 %. to as high as 200%. 5. 5. Analysis of function-staining electrophoresis indicated that even though the potency of the digestive proteases was not decreased, the composition of the enzymes had changed by the presence of three more isozymes. 6. 6. This also suggested that when monitoring enzyme systems of marine organisms with respect to osmolarity, the assay system should not be directly adapted from that of mammalian system but should be adjusted according to the physiological conditions of the organism.
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