Abstract
The effect of different concentrations of salicylic acid on total Kjeldahl nitrogen and nitrate reductase activity in the maize (Zea mays L.) seedling was studied. The total nitrogen of the maize embryonic axis (root + shoot) from seedlings raised with 10 mM Ca(NO3)2 for 5 days was substantially higher than that from the control when 0.01 mM salicylic acid was supplied. As supply of high (1 mM) concentrations of salicylic acid decreased the accumulation of organic nitrogen. The in vivo activity of nitrate reductase in the roots increased at low concentrations of salicylic acid, while high concentrations were inhibitory. The stimulative concentration of the acid protected in vivo loss of nitrate reductase activity under non‐inducing conditions, whereas it had no effect on in vitro loss of enzyme. It is suggested that salicylic acid increases in vivo enzyme activity indirectly, to some extent by protecting the natural inactivation of the enzyme.
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