Effect of salicylic acid on cowpea seedlings under saline stress

Abstract

The aim of this work was applying salicylic acid (SA) in cowpea seedlings under saline stress. The experiment took place in the seed laboratory of the Universidade Federal Rural da Amazônia with a completely randomized experimental design in a 2 x 2 x 3 factorial scheme, with two bean cultivars (Canapu and Pingo-de-ouro), two levels of salicylic acid (0, and 0.50 mM) and three salt stress levels (0, 25, 50 mM). The seeds were previously soaked in salicylic acid (0 and 0.50 mM) for a period of 12 hours and then placed in germitest paper rolls for treatments with NaCl (0, 25, 50 mM) for a period of 12 days at room temperature constant 27 °C. There was a significant effect of cultivars, AS dose and NaCl concentrations and their interactions on most of the analyzed variables. Root and leaf proline concentrations were higher in pingo-de-ouro cultivar, Canapu cultivar had better performance in biomass accumulation. Salicylic acid reduced proteins in the leaves by 13.33%, while in the root there was an increase of 12.61%, ammonium concentrations reduced in the roots by 11.9%. When applied to salinity (25 and 50 mM) there was an increase of proteins in the leaves 40.83% and 27.48% respectively, and a reduction of amino acids of 30.24 and 25.24% in NaCl dosages (25 and 50 mM) respectively. Salinity reduced biomass accumulation and interfered with cellular solute production. However, the application of salicylic acid promoted salt stress tolerance in Canapu cultivar.