Effect of Salicylic Acid and Nano-Silicon of the Enzymatic Activity for Potato Shoots Grown Under Salt Stress in Vitro

Abstract

The experiment was conducted in the tissue culture laboratory, Department of Horticulture and Landscaping, College of Agriculture, Al-Qasim Green University during the September 2021 to June 2022. The experiment was conducted according to a complete randomized design (CRD) with two factors and ten replicates. The first factor included sodium chloride in three concentrations (0, 50, 100) mmol.L-1, and the second factor included five concentrations of salicylic acid and nano-silicon (0, 1.5 mg.L-1 nano-silicon, 3.0 mg.L-1 nano-silicon and 14 mg.L-1 salicylic acid, 28 mg.L-1 salicylic acid). The results of the experiment showed the interaction between sodium chloride and addition of salicylic acid or nano silicon to the MS medium, and there was a significant effect of the enzymatic activity and proline. The interaction treatment ( 100 mmol.L-1 NaCl with salicylic acid14 mg.L-1) recorded the highest activity of Peroxidase (43.03), compared with the interaction treatment (0 mmol.L-1 NaCl with salicylic acid14 mg.L-1) which recorded the lowest activity of the Peroxidase enzyme reached (23.22). The interaction treatment ( 50 mmol.L-1 NaCl with salicylic acid at a 14 mg.L-1) recorded the highest activity of the enzyme (14.13).Compared with the interaction treatment (0 mmol.L-1 NaCl with 28 mg.L-1 salicylic acid), which recorded the lowest activity of the catalase enzyme (8.89). The interaction treatment (100 mmol.l-1 NaCl without adding nano-silicon or salicylic acid ) recorded the highest average of shoots content of proline (0.868). Compared with the interaction treatment (0 mmol.L-1 NaCl with 14 mg.L-1 salicylic acid), which recorded the lowest average in proline content (0.555).