Abstract
The platelet aggregation and 5-HT release by washed platelet from coronary heart disease patients following platelet activating factor (PAF) treatment were detected by turbidimetry and O-phthalaldehyde assay. The free calcium concentration in the platelets was measured with the fura-2/AM probe fluorescent technique. Results showed safflower yellow could inhibit the PAF induced washed platelet aggregation and 5-HT release, which were in a safflor-yellow-dose dependent manner. When the PAF was 2.0×10 -9 mol/L, the inhibition rate of platelet aggregation was 26.2%, 41.3%, 58.1%, 81.2%, and the inhibition rate of 5-HT release was 3.7%, 11.9%, 29.9% and 54.4% after treatment with safflower yellow at 0.21, 0.42, 0.85 and 1.69 g/L, respectively. The study concludes safflower yellow can inhibit the PAF induced platelet aggregation, 5-HT release by platelets and elevation of free calcium in platelets.
Highlights
Safflower is the dry flower of Cathamus tinctorius L which has been used in traditional Chinese medicine for promoting blood circulation for removing blood stasis
When the ginkgolide concentration was 2.10 × 10-2 pg/L, the inhibition rate of platelet aggregation was 91.3% and when the safflower yellow was at 1.6 pg/L, the inhibition rate of platelet aggregation was 81.2% (Table I)
Studies have confirmed that 1) safflower yellow can inhibit thrombosis, dilate blood vessels and combat with platelet aggregation to improve cerebral circulation; 2) safflower yellow can combat with free radicals and oxygenation, elevate the activities of superoxide dismutase and glutathione peroxidase, inhibit the lipid peroxidation and reduce the degradation of phospholipid in the membrane which protect cells and promote cellular metabolism; 3) safflower yellow can reduce the levels of cholesterol and triglyceride and improve the blood viscosity; 4) safflower yellow can exert neuroprotective effect which prevent ischemia/reperfusion injury, improve neurological function and attenuate brain edema (Jiang, 2011)
Summary
Safflower is the dry flower of Cathamus tinctorius L which has been used in traditional Chinese medicine for promoting blood circulation for removing blood stasis. To date, it has been applied in the treatment of trauma, gynecological diseases, cardiovascular diseases and pulmonary fibrosis (Wang et al, 2011). More than 200 compounds have been isolated from safflower including flavonoids, phenylethanoid glycosides, coumarins, fatty acids, steriods and safflower polysaccharides. Among these compounds, safflower yellow (SY) is the effective part of the aqueous extract of safflower (Fan et al, 2009). The SY for injection has been used to treat myocardial ischemia in clinical at 80 mg/d (Wang et al, 2011)
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