Effect of ruthenium complexes on the activities of succinate dehydrogenase and cytochrome oxidase

Abstract

In this article, we report the effects of acute administration of ruthenium complexes, trans-[RuCl 2(nic) 4] (nic = 3-pyridinecarboxylic acid) 180.7 μmol/kg (complex I), trans-[RuCl 2(i-nic) 4] (i-nic = 4-pyridinecarboxylic acid) 13.6 μmol/kg (complex II), trans-[RuCl 2(dinic) 4] (dinic = 3,5-pyridinedicarboxylic acid) 180.7 μmol/kg (complex III) and trans-[RuCl 2(i-dinic) 4]Cl (i-dinic = 3,4-pyridinedicarboxylic acid) 180.7 μmol/kg (complex IV) on succinate dehydrogenase (SDH) and cytochrome oxidase (COX) activities in brain (hippocampus, striatum and cerebral cortex), heart, skeletal muscle, liver and kidney of rats. Our results showed that complex I inhibited SDH activity in hippocampus, cerebral cortex, heart and liver; and inhibited COX in heart and kidney. Complex II inhibited SDH in heart and hippocampus; COX was inhibited in hippocampus, heart, liver and kidney. SDH activity was inhibited by complex III in heart, muscle, liver and kidney. However, COX activity was increased in hippocampus, striatum, cerebral cortex and kidney. Complex IV inhibited SDH activity in muscle and liver; COX activity was inhibited in kidney and increased in hippocampus, striatum and cerebral cortex. In a general manner, the complexes tested in this work decrease the activities of SDH and COX in heart, skeletal muscle, liver and kidney. In brain, complexes I and II were shown to be inhibitors and complexes III and IV activators of these enzymes. In vitro studies showed that the ruthenium complexes III and IV did not alter COX activity in kidney, but activated the enzyme in hippocampus, striatum and cerebral cortex, suggesting that these complexes present a direct action on COX in brain.