Effect of rosiglitazone on adhesion molecule expression of lung injury in rats with acute pancreatitis

Abstract

Objective To investigate the effect of rosiglitazone, the peroxisome proliferator-ac-tivated receptor-)' ligand, on adhesion molecule expression of lung injury in rats with acute pancreati-tis. Methods Fifty-four male Wistar rats were randomized into sham operation group (SO group), severe acute pancreatitis group (SAP group) and rosiglitazone pretreatment group (ROSI group). A-cute pancreatitis model was induced by retrograde infusion of 5% sodium taurocholate into the bilio-pancreatic duct. Rosiglitazone (6 mg/kg) dissolved in 10% DMSO was injected through the femoral vein 30 minutes piror to the operation. The rats of SO group and SAP group were injected partes ae-quales 10% DMSO. Rats were killed at 3, 6 and 12 h after operation. The serum amylase level, lung myeloperoxidase (MPO) activity and wet/dry ratio were determined. Lung tissue samples were stained respectively with hematoxylin and eosin for histopathological evaluation. Reverse transcription-polymerase chain reaction was used to detect the expression of ICAM-1, P-selectin and E-selectin mR-NA in lung tissue. Results The levels of amylase, MPO activity, wet/dry ratio and lung pathologic score were increased significantly in SAP group than in SO group (P< 0.05). Compared with SO group, the expression of ICAM-1, P-selectin and E-selectin mRNA were also increased significantly in SAP group (P<0.05). Pretreatment with rosiglhazone reduced amylase level, MPO activity, wet/ dry ratio and pathologic score at 6h and 12 h (P<0. 05), decreased the expression of ICAM-1, P-se-lectin and E-selectin mRNA at 12 h (P<0.05). Conclusion Rosiglitazone exerts the protective effect on acute pancreatitis associated lung injury through down-regulating the expression of ICAM-1, P-se-lectin and E-selectin mRNA. Key words: Pancreatitis; Peroxisome proliferator-activated receptor; Lung injury; Adhe-sion molecule