Effect of RNAi targeting CYP6CY3 on the growth, development and insecticide susceptibility of Aphis gossypii by using nanocarrier-based transdermal dsRNA delivery system

Abstract

RNA interference (RNAi) has been proved to be a viable method for agricultural pest control. Due to the limited uptake of dsRNA in hemiptera insects, this study used nanocarrier SPc (star polycation) transdermal delivery systems to deliver two truncated fragments (P1/P2) dsRNA of the CYP6CY3 for silencing this target gene in Aphis gossypii. After the cotton aphid was sprayed with the SPc + dsP1/P2 mixture, the expression level of target gene in SPc + dsP1 treatment group was not different from that in dsP1 group at 24 h, 48 h, and significantly lower than that in dsP1 group at 60 h, 72 h, respectively; and the expression level of target gene in SPc + dsP2 treatment group was not different from that in dsP2 group at 24 h, and significantly lower than that in dsP2 group from 48 h, 60 h, 72 h, respectively. In addition, the expression level was continuously silenced after spraying the SPc + dsP1/P2 mixture and significant reduced by 79.7% and 84.3% at 48 h compared with the H2O control group, the mortality rate reached 48.09% and 43.18% at 84 h, respectively. And the cumulative reproduction number of cotton aphids also decreased, but the cumulative death number of newborn nymphs had an increase trend, compared with the control groups. Bioassays after RNAi showed that the silencing of CYP6CY3 increased the susceptibility of the 4th instar aphid to imidacloprid, and increased mortality by 67.21% and 58.69% at 96 h, respectively. The life table parameters of the offspring from the 4th instar cotton aphids from the SPc + dsP1/P2 treatment groups showed that the offspring had a longer pre-reproductive period and post-reproductive period. The intrinsic growth rate was 0.231 ± 0.005, 0.210 ± 0.013 and the finite growth rate was 1.260 ± 0.007 and 1.234 ± 0.016 in the SPc + dsP1/P2 treatment group, these two parameters of the two groups were lower than that of the corresponding control，the population doubling time of the two groups was prolonged and the developmental duration was delayed. These results indicate that CYP6CY3 plays a key role in the growth, development, reproduction and detoxification ability in cotton aphids, and may be as a potential RNAi target for controlling aphids, laying the foundation for the development of new environmentally-friendly RNA pesticides in this field.