Abstract
Ribosome-inactivating proteins (RIPs) are a class of cytotoxic enzymes that can inhibit protein translation by depurinating rRNA. Most plant RIPs are synthesized with a leader sequence that sequesters the proteins to a cell compartment away from the host ribosomes. However, several rice RIPs lack these signal peptides suggesting they reside in the cytosol in close proximity to the plant ribosomes. This paper aims to elucidate the physiological function of two nucleocytoplasmic RIPs from rice, in particular, the type 1 RIP referred to as OsRIP1 and a presumed type 3 RIP called nuRIP. Transgenic rice lines overexpressing these RIPs were constructed and studied for developmental effects resulting from this overexpression under greenhouse conditions. In addition, the performance of transgenic seedlings in response to drought, salt, abscisic acid and methyl jasmonate treatment was investigated. Results suggest that both RIPs can affect methyl jasmonate mediated stress responses.
Highlights
We focus on two Ribosome-inactivating proteins (RIPs) from rice that are synthesized without a signal peptide
Data, OsRIP1 expression drought was upregulated whenstress, rice seedlings were posed to abscisic acid sequencing (ABA), jasmonate, cold, cadmium, and osmotic exposed to abscisic acid (ABA), jasmonate, cold, cadmium, drought and osmotic stress, while downregulation of OsRIP1 expression was observed when seedlings were exposed while downregulation of
The OsRIP1 sequence has recently been reported as a type 1 RIP, and the recombinant protein made in E. coli was proven to be enzymatically active on ribosomes from rabbits and insects [10]
Summary
RNA sequencing data have shown that rice RIPs are synthesized in response to both abiotic and biotic stresses [6,7]. Overexpression of OSjRIP7.2 (LOC_Os07g37090) in rice conferred resistance to drought and salt stress [8]. OsRIP1 (OsjRIP1.1, LOC_Os01g06740) was identified recently and was shown to represent a nucleocytoplasmic type 1 RIP with proven enzymatic activity [10]. Based on RNA data, OsRIP1 expression drought was upregulated whenstress, rice seedlings were posed to abscisic acid sequencing (ABA), jasmonate, cold, cadmium, and osmotic exposed to abscisic acid (ABA), jasmonate, cold, cadmium, drought and osmotic stress, while downregulation of OsRIP1 expression was observed when seedlings were exposed while downregulation of. The sequence of nuRIP (OsjRIP11.2, LOC_Os11g06460) has a high degree of similarity to the type 3 RIP from barley, called JIP60. Plants when exposed to drought, salt, abscisic acid (ABA) and methyl jasmonate (MeJA)
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