Year-end Sale % OFF 
Abstract
Objective To investigate the effects of Ras homolog gene family,member E (RhoE)overexpression on the apoptosis of human glioma U251 cells and the mechanism.Methods The Myc-N1 and Myc-RhoE plasmids were transfected into human glioma U251 cells by Lipofectamine technology.The changes of RhoE and Cyclin D1 in transfected glioma cells were detected by reverse transcriptase-polymerase chain reaction (RT-PCR) and Western blotting.The cell proliferaion and apopotosis were analyzed by methyl thiazol tetrazolium (MTF) assay and flow cytometry.Results The RhoE mRNA and protein levels in RhoE-U251 group was significantly up-regulated compared to N1-U251 group and control group (P ＜0.05),while the Cyclin D1 mRNA and protein levels in RhoE-U251 group were significantly suppressed.After overexpression of RhoE was up-regulated,the growth of these cells was inhibited and their apoptosis was obviously enhanced.Conclusion RhoE may be a tumor suppressor gene.Up-regulation of RhoE expression can down-regulate the Cyclin D1 expression,and then promote the apoptosis and inhibit the growth of human glioma U251 cells. Key words: Glioma; Apoptosis ; RhoE ; Cyclin D1
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
