Effect of Rhizopus azygosporus UICC 539 growth on the nutrient content of sterile slurry and palm kernel cake mixtures at different temperature

Abstract

This study reported the effect of Rhizopus azygosporus UICC 539 growth on the sterile slurry and palm kernel cake (PKC), and analysis of the nutrient content of the waste mixtures. The fungus showed good growth on Potato Sucrose Agar (PSA) at temperature range of 30 to 50 °C, and could not grow at 55 °C. Inoculum was prepared from fungal culture in Potato Sucrose Broth (PSB) by still fermentation at 30 and 40 °C for 5 days. Sterile slurry and PKC (3:1) in Petri dishes (diameter 9 cm) were prepared and solid-state fermentation was carried out using inoculum (10 %, v/v) at 30 and 40 °C, and incubated for 5 days. Rhizopus azygosporus UICC 539 showed good growth and increased cell numbers on sterile waste mixtures. Effect of fungal growth on the nutrient content of the waste mixtures was observed by comparing the treatment and control. There was an increase in the moisture and ash content, and a decrease in protein content, total calorie and carbohydrate content. There was no change of the calorie from fat and total fat content compared to the control.