Effect of Rhizobacterein Bio Fertilizer, Nano-NPK and Nano-Micro Complete on some Parameters of Growth and Yield of Bean and Number of Bacteria in the Rhizosphere

Abstract

During the winter season 2021-2022, an experiment was carried out in the wooden canopy of the College of Agriculture and Forestry / University of Mosul to study of effect of Rhizobacterein, Nano-NPK20:20:20, and Nano-Micro Complete fertilizers and time periods on the number of rhizosphere bacteria and some of the growth and yield parameters of bean. Using (Silt Loam) texture in Alhawi soil in Mosul region. The first factor contained nine treatments of (NPK20:20:20), (Micro Complete) nano-fertilizer, Rhizobacterein bio-fertilizer, dual and tri combination of them, In addition to a treatment containing conventional fertilizers, as well as a control. The second factor is the time periods (60, 90 and 120) days of cultivation using a complete random design (CRD) with three replications. On 24/11/2021, the seeds of the Italian-origin bean Luz cultivar were planted in polyethylene (10) kg of soil. Fertilizers were combined with the soil and added in two batches, the first after germination and the second 20 days later. For each time, the total number of bacteria and vegetative properties were counted. (60, 90, and 120) cultivation days. The results of the statistical analysis for the least significant difference (L.S.D0.05) showed superiority of the tri combination of fertilization (Nano-NPK+Nano-Micro complete+Rhzobacterein) for the period (120) days after planting in the chlorophyll index, fresh pods yield, and fresh plants yield (51.84 SPAD, 71.62 g plant−1, and 33.85 g plant−1) respectively, compared to the same combination of the traditional sources (49.2 SPAD, 62.86 g plant−1, and 24.72 g plant−1) for the same period, while the same trend was achieved with the total numbers of bacteria for the tri nano, bio fertilizers combination (6.93, 10.12, 5.03) (108) cfu g−1soil, compared to the tri traditional, bio fertilizers combination (5.14, 6.73, 4.59) (108) cfu g−1 soil, respectively for the periods (60, 90, and 120) days after of planting.