Abstract
Objective To investigate the effects of the peptides(RGDS-GG-KLAKLAKK)on the growth of human osteosarcoma cells(MG-63).Methods The MG-63 cells were co-cultured with different concentrations of RGDS-GG-KLAKLAKK(0,25,50,75,100 μmol/L).The suppressive effect of RGDS-GG-KLAKLAKK on MG-63 cells was examined by MTr assay.The apoptosis of MG-63 ceils was observed by flow cytometry(FCM).The Survivin mRNA levels were detected by RT-PCR.Results RGDS-GG-KLAKLAKK obviously suppressed the proliferation of MG-63 cells in both time-and dose-dependent manners,and the IC50 was 46.8,37.6 μmol/L at 24 and 48 h respectively(P ＜ 0.05).The FCM results indicated that apoptosis ratio of MG-63 cells was increased from 5.23% of untreated cells to 38.90% of treated cells(P ＜0.01).RT-PCR revealed that the expression of Survivin mRNA in MG-63 cells was down-regulated.Conclusion The RGDS-GG-KLAKLAKK can suppress the proliferation of MG-63 cells,and induce their apoptosis. Key words: Peptide; Osteosareoraa; Survivin
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
