Effect of resveratrol on myocardial energy metabolism in sepsis rats

Abstract

To investigate the influence of resveratrol (RES) on myocardial energy metabolism in sepsis rats and its mechanism. Twenty Sprague-Dawley (SD) rats were divided into four groups according to the random number table, 5 rats in each group. The sepsis model was reproduced by intraperitoneal injection of lipopolysaccharide (LPS) 10 mg/kg, and the rats in sham group were injected intraperitoneally with the same amount of saline. The rats in RES treatment group (RES group) were injected RES 8 mg/kg 10 minutes before LPS injection, and those in RES combined with the silent information regulator 1 (sirti) inhibitor group (RES + sirtinol group) were injected sirtinol 1 mg/kg followed by RES 8 mg/kg 10 minutes before LPS injection. The rats were sacrificed 6 hours later, and the heart was harvested. The myocardial ultrastructure was observed by transmission electron microscope. The expression of sirt1 protein was detected by Western Blot. The cytochrome C oxidase (CCO) activity was determined by ultraviolet spectrophotometry. The content of adenosine triphosphate (ATP), adenosine diphosphate (ADP), and adenosine monophosphate (AMP) were detected by high-performance liquid chromatography (HPLC). Under transmission electron microscopy, the mitochrondria in myocardium in sham group was normal, and mitochrondria was swollen and derangement in LPS group and RES + sirtinol group, but it was not significant in RES group. Compared with sham group, the siit1 protein expression in LPS group was decreased (gray value: 0.602 ± 0.038 vs. 0.902 ± 0.037, P < 0.05), the CCO activity was declined (U/mg: 0.344 ± 0.019 vs. 0.600 ± 0.023, P < 0.05), the contents of ATP, ADP, and AMP were decreased [ATP (µg/g): 89.958 ± 7.570 vs. 157.460 ± 6.737, ADP (µg/g): 164.658 ± 6.742 vs. 251.608 ± 5.191, AMP (µg/g): 179.926 ± 7.303 vs. 276.262 ± 5.546 all P < 0.05]. Compared with LPS group, the sirt1 protein expression in RES group was increased (gray value: 0.874 ± 0.017 vs. 0.602 ± 0.038, P < 0.05), the CCO activity was increased (U/mg: 0.574 ± 0.013 vs. 0.344 ± 0.019, P < 0.05), the contents of ATP, ADP, and AMP were increased [ATP (µg/g): 97.942 ± 11.257 vs. 147.686 ± 8.853, ADP (µG/G): 168.888 ± 8.965 VS. 245.860 ± 7.111, AMP (µg/g): 175.498 ± 6.993 vs. 271.260 ± 6.658, all P < 0.05].