Effect of resuscitation with Ringer′s malate solution on acute lung injury caused by hemorrhagic shock in rats

Abstract

Objective To evaluate the effect of resuscitation with Ringer′s malate solution on acute lung injury caused by hemorrhagic shock in rats. Methods Forty-eight SPF healthy male Sprague-Dawley rats, aged 7-9 weeks, weighing 280-320 g, were assigned into 4 groups (n=12 each) using a random number table method: sham operation group (group S), normal saline group (group NS), Ringer′s lactate solution group (group RL) and Ringer′s malate solution group (group RM). In NS, RL and RM groups, the model of hemorrhagic shock was established, and rats were resuscitated after 60 min of hemorrhagic shock.Rats were sacrificed at 3 h after resuscitation, and bronchial alveolar lavage fluid (BALF) was collected to count neutrophils.Lung tissues were obtained for determination of the wet/dry weight ratio (W/D ratio), myeloperoxidase (MPO) activity, and contents of tumor necrosis factor-alpha (TNF-α), interleukin-1beta (IL-1β) and IL-6.Lung tissues were obtained for examination of the pathological changes. Results Compared with group S, the neutrophil count in BALF, W/D ratio, MPO activity and contents of TNF-α, IL-1β and IL-6 were significantly increased in NS, RL and RM groups (P<0.05). Compared with NS and RL groups, the neutrophil count in BALF, W/D ratio, MPO activity and contents of TNF-α, IL-1β and IL-6 were significantly decreased in NS and RL groups (P<0.05). Conclusion The severity of acute lung injury is reduced when Ringer′s malate solution is used for resuscitation as compared with that when normal saline and Ringer′s lactate solution are used in a rat model of hemorrhagic shock. Key words: Malates; Shock, hemorrhagic; Fluid therapy; Respiratory distress syndrome, adult