Effect of residues remaining in the injection liner of a gas chromatograph on the quantification of polycyclic aromatic hydrocarbons by isotope dilution mass spectrometry using deuterium-labeled internal standards

Abstract

We have investigated the differences in response ratios of native polycyclic aromatic hydrocarbons (native PAHs)/ 13C-labeled PAHs ( 13C-PAHs) and of native PAHs/deuterium-labeled PAHs (PAHs- d) in a calibration solution containing at trace concentrations (<1 μg mL −1) by gas chromatography–mass spectrometry. Only the ratios of native PAHs/PAHs- d decreased significantly ( p < 0.05) accompanied with increase of residue amounts originated from immediately before injected solution. Since the analytical results using PAHs- d as internal standards were significantly lower than those using 13C-PAHs whatever solutions were injected immediately before ( p < 0.05, 1.9–13.2%), 13C-PAHs gave more accurate results.