Effect of replication-defective herpes simplex virus mediated kynurenine aminotransferase on detrusor overactivity in rats with spinal cord injury

Abstract

Objective To evaluate if replication-defective herpes simplex virus mediated kynurenine aminotransferase Ⅱ (HSV-KATⅡ) could inhibit detrusor overactivity (DO) in rats with spinal cord injury by bladder wall injection. Methods From June 2012 to July 2013, 48 male Wistar rats with T10 spinal cord transection (SCT) were randomly divided into normal saline group, HSV group and HSV-KATⅡ group (n=16 each group). Normal saline (40 μl), HSV or HSV-KAT Ⅱ(40 μl, 1×107 plaque forming unit viruses) was injected into the rat bladder wall of according group by 1 week after SCT. Three weeks after injection, cystometry was performed and the virus transfected efficiency, expression of KATⅡ in L6–S1 dorsal root ganglia were examined. Results The DO number, DO amplitude, maximum voiding pressure and volume inducing voiding were decreased significantly by 59.6%–61.1%, 21.6%–24.2%, 30.3%–34.4% and 44.1%–46.5% (P 0.05). The relative intensity of KATⅡ protein (0.50±0.13 versus 0.28±0.07, P<0.05) and mRNA (0.78±0.06 versus 0.51±0.08, P<0.01) were increased significantly in HSV-KATⅡgroup than those in HSV group. Conclusion HSV-KATⅡ bladder wall injection inhibits DO and may improve detrusor-sphincter dyssynergia in rats with spinal cord injury. Key words: Spinal cord injuries; Detrusor overactivity; Herpes simplex virus; Kynurenine aminotransferase; Rats, Wistar