Abstract
Objective To investigate the effect of repeated ketamine administration ou memory ability of infant rats and the possible mechanism.Methods Sixty-four SD rats of 21 days old were divided into ketamine group(including two subsets:k1 group and k7 group.The rats were anesthetized with 75 mg/kg ketamine per day for 7 times,and were trained respectively at one day and seven days after the last injection.Some rats of the two groups were killed at one hour after the first traning,and others were trained again at three days after the first traning),normal saline group(the rats were given equal volume of 0.9%saline),pseudotraining group(the rats were trained according to the average traning time and times of normal saline group and were killed at one hour after the training)and passive control group(the rats were killed after 5 minutes staying in the Y-maze).Y-maze was used to test the memory ability.P-CREB protein was tested by immunhistochemistry.Results The traning time and times in k1 group[(53.38±19.03)min,(51.88±15.93)times]were higher than those in normal saline group [(21.88±8.11)min,(25.50±6.78)times](P＜0.05).The memory consolidation rate and the p-CREB protein expressing cells of k1 group[(61.25±6.41)%,(28.62±2.92)]were fewer than those in normal saline group [(87.50±13.89)%,(71.13±7.23)](P＜0.05).There were not significantly different between k7 group [(22.75±7.65)times,(24.13±9.01)min,(87.50±10.35)%,(71.00±6.93)]and normal saline groupThe number of p-CREB expressing cells was greater in nornlal saline group and ketamine group than that in passive control group[(7.75±1.28)],which was not significantly different between pseudotraining group[(7.75±1.49)]and passive control group.Conclusion Administration of repeated ketamine may temporarily affect the memory ability of infant rats through suppressing the expression of p-CREB protein in the hippocampus. Key words: Ketamine; Infant; Learning and memory; Hippocampus; p-CREB
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