Abstract
Objective To explore the effect of valsartan on pancreas microcirculation in rats with severe acute pancreatitis (SAP) and the action mechanism.Methods The SAP rats were randomly assigned to 3 groups:the control group (SO group),model group (SAP group) and valsartan-treated group (Ⅴ group).The rat model of SAP was established by retrograde injection of 5％ sodium taurocholate into the biliopancreatic duct.SO group was subjected to bile duct retrograde injection of the same amount of physiological saline,and Ⅴ group to femoral vein injection of valsartan 10 min after modeling.The changes in serum amylase (AMY),pathology,angiotensin (Ang) Ⅱ,malondialdehyde (MDA),myeloperoxidase (MPO),intercellular adhesion molecule-1 (ICAM-1),and P-selectin in pancreatic tissues were observed.Results At 3,6 and 12 h after operation in SAP group,the contents of AMY were (6149.7 ± 393.1),(7401.4 ±578.5) and (9142.9 ± 751.8) U/dl,the contents of MDA were (4.42 ± 1.21),(5.61 ±1.17) and (5.95 ± 1.12) μmol/L,and the levels of MPO were (3.28 ±0.64),(4.62 ±0.87) and (5.22 ±0.75) U/g,the contents of AngⅡ were (240.90 ±31.46),(273.96 ±27.85) and (355.72 ±42.77) ng/L,which were significantly higher than in SO group [for AMY:(3845.8 ± 304.0),(4462.6±412.9) and (4898.0 ±408.2) U/dl; for MDA:(3.64 ±0.82),(3.79 ±0.71) and (3.94±1.03) μmol/L; for MPO:(1.94±0.72),(2.31 ±0.81) and (3.18±0.73) U/g; for AngⅡ:(124.33 ±15.72),(162.42 ± 18.81) and (220.28 ± 19.73) ng/L] (all P＜0.05).The P-selectin espresssion levels (23％) and the levels of ICAM-1 in Ⅴ group were significantly lower than in SAP group (P ＜ 0.05),but higher than in SO group (P ＜ 0.05).The pancreatic injury in SAP group was aggravated as compared with Ⅴ group.Conclusion The Ang Ⅱ contents in the SAP rats were significantly increased in the pancreatic tissue.Valsartan had a protective effect on the pancreas of SAP rats probably by inhibiting the contents of MDA and MPO,and the expression levels of ICAM-1 and P-selectin. Key words: Acute pancreatitis; Angiotensin Ⅱ; Intercellular adhesion molecule-1; P-selectin
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