Abstract
Sperm motility was estimated by a transmembrane migration ratio (TMMR) method, which is based on the proportion of sperm that migrate across a membrane containing micropores with a diameter slightly greater than the width of the sperm nucleus. Relaxin (1000, 333 and 167 ng/ml) attenuated ( P < 0.01) loss of sperm motility during 120 min (50 vs 40%). The addition of 1000 ng/ml relaxin to semen improved sperm motility ( P < 0.05) stored at 12°C for 5 days with compared controls (43 vs 32%). Antirelaxin serum (R6) and normal rabbit serum (NRS; 1:9000) were added to semen and the sperm motility was determined at 0, 10, 40, 80 and 120 min. Antirelaxin serum abruptly inhibited sperm motility ( P < 0.01) within 10 min compared with NRS-treated control semen (25 vs 58%). Caffeine and relaxin stimulated motility ( P < 0.01) of frozen-thawed porcine semen; however, relaxin maintained greater sperm motility ( P < 0.01) than caffeine after 80 min (30 vs 16%). These results indicate that purified porcine relaxin can maintain or increase sperm motility; in contrast, porcine antirelaxin abruptly inhibits sperm motility.
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