Abstract
BackgroundVietnamese medicine tried and tested certain bioactive compounds from plants to increase the rate of tissue immunomodulation, regeneration, and differentiation. Although there are many research papers discovered about phytochemicals of Rehmannia glutinosa Libosch and differentiation induction potential of some substances purified from this herbal, it finds difficult to seek research that investigated the effect of hot water-extracted R. glutinosa Libosch (RGE) on proliferation and cardiogenic differentiation of mesenchymal stem cells, even though it has commonly been used for a long time because of its function as a restorative and as a critical role in cardiovascular treatment in traditional.ResultsOur research indicated that RGE has many predicted bio-pharmacological effects, and the RGE is demonstrated that it is non-toxic to UC-MSCs (IC50 = 1274 ppm). It also stimulates the proliferation and migration of UC-MSCs at various concentrations, especially at the RGE concentration of 50 ppm, during four days of treatment. On the other hand, the RGE can induce the cardiac pre-differentiation process from the fifth day to the fifteenth day after treatment, which was proven through both molecular and cellular (morphology evidence) levels like the up-regulation of GATA4, Nkx2.5, cTnT α-MHC, Desmin genes; the expression of Desmin protein, the appearance of two-nuclei cells, connecting process of adjoining cells, the cytoplasmic striations.ConclusionThe RGE could either stimulate proliferation–migration of MSCs or induce the cardiac pre-differentiation process. This extract can be classified as non-toxic to the UC-MSCs.
Highlights
Rehmannia glutinosa Libosch (RG) is a carbohydraterich plant in the family of Scrophulariaceae and is a famous folkloric medicinal plant in Vietnam
Research works in the past revealed that RG contains many components in its extract [1], which is responsible for various pharmacological effects such as treatment potential on osteoporosis [2], hypoglycemia in various diabetic disorders [3], antisenescence [4], cardiovascular diseases [5], heart failure [6], and tumor [7]
There is a lack of information and knowledge about the impact of hot water-extracted R. glutinosa Libosch on mesenchymal stem cells even though residents of Eastern countries often use RG with a form like crude extract instead of using the purified individual components
Summary
Rehmannia glutinosa Libosch (RG) is a carbohydraterich plant in the family of Scrophulariaceae and is a famous folkloric medicinal plant in Vietnam. The use of bone marrow-derived mesenchymal stem cells (BMSCs) has been popular in research and clinical studies, umbilical cord mesenchymal stem cells (UC-MSCs) can become an alternative source to BMSCs due to non-invasive collection protocol and easy accessibility. They have emerged strongly to the attractive therapy for many. There are many research papers discovered about phytochemicals of Rehmannia glutinosa Libosch and differentiation induction potential of some substances purified from this herbal, it finds difficult to seek research that investigated the effect of hot water- extracted R. glutinosa Libosch (RGE) on proliferation and cardiogenic differentiation of mesenchymal stem cells, even though it has commonly been used for a long time because of its function as a restorative and as a critical role in cardiovascular treatment in traditional
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