Effect of regulating qi and invigorate blood circulation method on hemorrheology in rats with acute blood stasis syndrome and its network pharmacology research

Abstract

To observe the effect of regulating qi and invigorate blood circulation method on hemorrheology in rats with acute blood stasis syndrome from the perspective of modern pharmacology, and use network pharmacology to explain its mechanism. Forty male Wistar rats were randomly divided into 6 groups. Except the blank control group, the other groups were injected with adrenaline hydrochloride and ice bath to establish the acute blood stasis syndrome model. Each group were administered Xuefu Zhuyu D ecoction, Jinlingzi Powder, Taohong Siwu Decoction, and Yanhusuo Decoction, and t he blank control group and model control group were given the same volume of saline, respectively. The blood rheology indexes such as red blood cell aggregation index of rats were measured. The TCMSP and Batman database were used to search for chemical components and targets of Jinlingzi Powder, and protein interaction analysis, GO enrichment analysis and KEGG analysis were performed on the common targets. The experimental results show that, compared with the model group, the blood rheology index levels of the Yanhusuo group and Taohong Siwu Decoction group are reduced, while the blood rheology index levels of the Xuefu Zhuyu Decoction group and the Jinlingzi S an group are significantly reduced. The network pharmacology predicts that Jinlingzi Powder has 44 active ingredients and 34 drug-disease shared targets, and KEGG analyzes 61 signaling pathways. Conclusion: IN the case of blood stasis syndrome, the effect of regulating qi with invigorate blood circulation is better than that of regulating qi alone. Network pharmacologic prediction showed that TNF signaling pathway was strongly correlated with hemorheology, and its action pathway was closely related to key tar gets IL6, TNF, CASP3.