Effect of reduced N6-Benzyladenine, explant type, expiant orientation, culture temperature and culture vessel type on regeneration of adventitious shoot and in vitro plantlets ofSpilanthes acmella

Abstract

Nodal expiants ofSpilanthes acmella produced normal multiple shoots when cultured vertically on Murashige and Skoog medium (1962) supplemented with 0.5 mg IT1 BA. The number of shoots formed from each expiant was doubled after first 5-week subculture. The nodal expiants placed vertically in Erlenmeyer flask (250, 500, or 1000 mL) produced more multiple shoots than those cultured in 350 mL jam bottles and 500 mL tex-Z flask. Temperature above 28C caused abnormalities of in vitro plantlets. All in vitro plantlets survived after acclimatized and transferred to the outside environment The survived plantlets did not show any morphological abnormalities in the field condition.