Effect of recombinant adenovirus encoding human p53 tumor suppressor gene(rAd-p53) on the growth and radiotherapeutic sensitivity of human lymphoma cell lines

Abstract

Objective To explore the inhibitory effect and radiation sensitization of recombinant adenovirus encoding human p53 tumor suppressor gene(rAd-053)on human lymphoma cell lines.Methods Human lymphoma cell lines Raji and Daudi were treated with rAd-p53.radiation therapy and combined treatment,respectively.the cell growth inhibition was assessed by MTT.The p53 protein expression was detected bv Western blotting, and p53 mRNA was detected by RT-PCR.Results The MTT results showed that the inhibitory effect and radiosensitivity enhancement of rAd-p53 on human lymphoma cell lines were not obvious [Raji:(27.5±4.1)%;Daudi:(28.1±1.6)%].The results of Western blotting and RT-PCR showed that extrinsic p53 protein and p53 mRNA were expressed to some degree,but not at high-level.In addition,the results didn't demonstrate obvious radiosensitivity enhancement.Conclusions The role of inhibition and .radiosensitivity enhancement of rAd-p53 was not significant on human lymphoma cell lines. Key words: rAd-p53; p53 gene; Human lymphoma cell lines; Radiation therapy