Effect of reabsorption on the concentration dependence of fluorescence lifetimes of chlorophyll a

Abstract

The concentration dependence of mean fluorescence lifetimes of chlorophyll a in methanol was studied in the concentration interval 3 × 10 −6 − 7.3 × 10 −5 mol l −1 at room temperature. The values found experimentally were corrected for reabsorption of fluorescence using the average molar absorptivity ϵ F derived from spectral properties of overlapping fluorescence and absorption spectra of chlorophyll a.