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Abstract
The aim was to study the effect of rabbit immunization with different yeasts cells on overall antimicrobial serum activity and specific activity of antimicrobial peptides fraction.Material and methods. Rabbits were immunized with cells of Candida albicans, Rhodotorula mucilaginosa, Malassezia furfur, Cryptococcus neoformans, Geotrichum candidum, Trichosporon cutaneum and Saccharomyces cerevisiae. AMP fractions were obtained by filtration through 100 kDa filters. Overall activities of sera and their AMP-fractions were estimated spectrophotometrically.Results. Overall serum antimicrobial activity had tendency to decrease after immunization independently from yeast species. Activity of AMP-fractions after immunization with C. albicans, Cr. neoformans, G. candi dum и S. ce revisiae increased, but with other yeasts — decreased. The highest sensitivity to AMP fraction cor res ponded to C. albicans, especially for serum of the rabbit immunized with this yeasts — 1,5-fold higher com pare to control. Serum albumin concentration showed the positive correlation with overall antimicrobial activity against C. albicans (r = 0,760), R. mucilaginosa (r = 0,728), M. furfur (r = 0,723) и T. cutaneum (r = 0,588).Discussion. The results confirm our earlier data. Moreover, they expand current views on the effect of yeasts antigens on activity of serum AMP against different yeasts.Conclusion. Serum albumin and serum AMP demonstrated the specificity toward different yeasts: maximal activity they showed towards specia which more often play opportunistic role.
Highlights
Immunization of mice with yeasts antigens accompanied by increase of antimicrobial peptides (AMP) fraction activity towards Candida albicans
Rabbits were immunized with cells of Candida albicans, Rhodotorula mucilaginosa, Malassezia furfur, Cryptococcus neoformans, Geotrichum candidum, Trichosporon cutaneum and Saccharomyces cerevisiae
The highest sensitivity to AMP fraction corresponded to C. albicans, especially for serum of the rabbit immunized with this yeasts — 1,5-fold higher compare to control
Summary
Арзуманян В.Г. , Иксанова А.М., Артемьева Т.А., Бутовченко Л.М. ФГБНУ «Научно-исследовательский институт вакцин и сывороток имени И.И. Установлено, что иммунизация мышей дрожжевыми антигенами сопровождается повышением активности фракции антимикробных пептидов (АМП) по отношению к Candida albicans. Цель работы — изучить влияние иммунизации кроликов разными видами дрожжей на общую антимикробную активность сыворотки и специфическую активность фракции ее АМП. Общую активность сывороток и их АМП-фракций оценивали спектрофотометрически. Активность АМП-фракций под действием C. albicans, Cr. neoformans, G. candidum и S. cerevisiae возрастала, а под действием прочих — снижалась. Наиболее высокая чувствительность к АМП-фракции сывороток соответствовала C. albicans, особенно в отношении сыворотки кролика, иммунизированного этими же дрожжами, — в 1,5 раза выше контроля. Результаты экспериментов не только согласуются с полученными ранее данными, но и расширяют представления о воздействии дрожжевых антигенов на активность сывороточных АМП против разных видов дрожжей. Для цитирования: Арзуманян В.Г., Иксанова А.М., Артемьева Т.А., Бутовченко Л.М. Влияние иммунизации кроликов дрожжевыми антигенами на активность фракции сывороточных антимикробных пептидов.
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