Abstract
Arbutus unedo L. is a Mediterranean species used for fruit production; it is tolerant to drought and shows regeneration ability following forest fires. Mycorrhizal plants with Tuber borchii add resilience and value. This study aims to test the effect of quercetin on mycorrhizal synthesis between T. borchii and A. unedo. Two genotypes selected for fruit production and hydric stress tolerance, were micropropagated for mycorrhizal synthesis, accomplished during ex vitro rooting in perlite, using lyophilized spores of T. borchii suspended in culture media with different quercetin levels (0–10 µM). Six months after inoculation, plants were transferred to pots and maintained in nursery. Ten and 12 months after inoculation, roots were morphological examined and molecularly characterized using ITS1-5.8SITS2 rDNA region and specific primers. Results showed that mycorrhizae establishment was dependent on studied factors (genotype, quercetin level, and culture medium) and their interaction (genotype X culture medium). Quercetin levels up to 2.0 µM favored mycorrhizae establishment and plant growth, although levels superior to 4 µM showed a toxic effect. Quercetin showed to be an efficient factor on inducing mycorrhiza thriving independent of the genotype. Morphological observations and molecular analysis confirmed the permanence of the fungus association 10 and 12 months after inoculation.
Highlights
Arbutus unedo L. is a Mediterranean species that can be found across Iberian Peninsula, southern Europe (Mediterranean basin countries), northernAfrica, and Ireland [1,2,3]
Six months after inoculation, when in vitro plants were transferred to nursery pots for the first time, root systems were examined for mycorrhizal formation
Secondary roots roots
Summary
Ireland [1,2,3] It is widely spread across Portugal and can grow from 20 to 1000 m in altitude, withstanding drought and degraded soils [4]. It is commonly found on the understory of oaks, Quercus suber on the south and Quercus robur on the north, as well as on pine stands. A. unedo has been recognized as an interesting species for economic and ecological purposes, such as its great ability to survive wildfires, due to sprouting, even if let without a full crown.
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
