Effect of pyridalyl on mortality, fecundity and physiological performance of olive fruit fly, Bactrocera oleae Rossi (Diptera: Tephritidae)

Abstract

Abstract Toxicity and physiological effects of pyridalyl were studied on the adults of Bacterocera oleae via oral exposure and biochemical experiments. First, adults were fed on a protein hydrolysate diet which has been amended by five concentrations of pyridalyl. Then, another cohort of flies was fed on LC50 concentration to find potential effects on fecundity, detoxifying enzymes and intermediary metabolism. Results of bioassay showed a concentration of 0.517 μg/ml as the LC50 value with confidence limit of 0.305–0.791 μg/ml at 95%. Fecundity of the females fed on pyridalyl-treated diet showed no significant differences with controls following 7 days of exposure while a significant higher mortality was recorded in laid eggs by the treated females. Activities of esterase and glutathione S-transferase significantly increased in the treated adults compared to control when α-naphthyl acetate and CDNB (1-chloro-2,4-dinitrobenzene) were used as substrates. Activities of alanine- and aspartate aminotransferases significantly increased in the pyridalyl-treated adults compared to control while no significant difference was observed in activity of ɤ-glutamyl transferase. Although lactate dehydrogenase showed no statistically different activity but both acid- and alkaline phosphatases had statistically higher activity in the control adults compared to treatment. Finally, low density lipoprotein and total protein were the only non-enzymatic components which showed statistical difference between control and treated flies. Our results indicated that pyridalyl had toxicity on B. oleae and intervened in the physiological performance of flies.