Effect of puromycin and actinomycin D on glucose-stimulated protein and RNA labeling, in vitro, in rat testis

Abstract

1. Glucose (9 mM) increases testicular RNA and protein labeling, in vitro. 2. In the absence of virtually all incorporation of uridine into RNA, glucose is still capable of enhancing testicular protein synthesis. 3. Conversely, in the absence of virtually all protein synthesis, glucose is still capable of stimulating incorporation of uridine into RNA. 4. It is concluded that the stimulatory effect of glucose on rat testicular protein and RNA labeling, in vitro, are independent phenomena. Furthermore, the stimulatory effect of glucose on protein labeling appears to involve primarily enhancement of protein synthesis per se, while the effect on RNA labeling appears to be principally the result of increased tissue uptake of the precursor.