Effect of purified zearalenone with or without modified montmorillonite on nutrient availability, genital organs and serum hormones in post-weaning piglets

Abstract

The aims of the present study were to investigate the toxicity of zearalenone (ZEA) on nutrient availability, genital organs and serum hormones of piglets, and to evaluate the efficacy of modified montmorillonite clay (Calibrin-Z) in preventing ZEA-induced adverse effects. The experiment was conducted for 22d using 36 piglets weaned at 21d of age (Landrace×Yorkshire×Duroc, 18 females and 18 males; 8.84±0.21kg average body weight). Piglets of each gender were randomly allocated to the following six dietary treatments: 1) Control (basal diet only); 2) Control+1g/kg clay; 3) Control+1mg/kg ZEA; 4) Control+1mg/kg ZEA+1g/kg clay; 5) Control+1mg/kg ZEA+2g/kg clay; and 6) Control+1mg/kg ZEA+4g/kg clay. Piglets were housed and fed individually for the entire experimental period. Blood samples were taken and piglets were sacrificed at the end of the experiment to obtain organs for physiological assessment. Results showed that treatments had no effect on average daily gain (ADG), average daily feed intake (ADFI), feed efficiency (FE, g gain/g feed) and apparent digestibility of nutrients. Females fed ZEA-contaminated diet without clay showed increase (P<0.05) in vulva size, relative weight of genital organs and proliferative changes of the corpus uteri tissues. However, the decreased relative weight of genital organs (P<0.05) in males were observed without difference in the testicle size (P=0.235) and histological changes. Serum progesterone (PG) and testosterone (Ts) in males and females, and estradiol (E2) in females were significantly decreased by the additional ZEA in feeds (P<0.05). Dietary addition of clay in the ZEA-contaminated (1.05mg/kg) diet showed a positive protection effect on the nutrient availability of GE and CP, net protein utilization (NPU), relative weight of genital organs in piglets, proliferative changes of the corpus uteri tissues in females, and serum hormones of PG and Ts in males and females, and E2 in females. However, piglets fed the control diet with clay alone at 1g/kg level had no impact on any of the parameters as compared to the control. It suggested that feeding ZEA at about 1.05mg/kg can result in a deleterious effect on piglets, which was totally or partly ameliorated by dietary supplementation of modified calcium montmorillonite clay at concentrations between 1 and 4g/kg diet.