Effect of pulsed millisecond current magnetic field on the proliferation of C6 rat glioma cells

Abstract

ABSTRACTIn recent years, using electromagnetic fields as a targeted therapy for tumors has become a new idea. This paper aims to study the response of rat glioma cells (C6) when the external electromagnetic field parameters change and to obtain a complete working range of magnetic field parameters. Four-day, 4-h daily millisecond magnetic field exposure experiments were performed with C6 cells. The peak values of magnetic field intensity were 260 mT, 90 mT, 19 mT and 6 mT. Each day after exposure, cell morphology and cell viability assay (MTT method) were measured. The response of C6 cells shows a significant window effect and time cumulative effect on the cell, and it is non-destructive. The working inhibited magnetic field range of magnetic field increase rate dB/dt (T/s) is [34, 119.5] and [166.75, 527.25], the magnetic field amplitude B (mT) is [6, 260], the magnetic field integral Bt (mT·s) is [0.1649, 0.8085] and the energy integral B2t (mT2·s) is [2.317, 53.328]. Our findings provide the theoretical and experimental basis for clinical applications of electromagnetic fields.