Effect of pulmonary surfactant on the cell cycle distribution and expression of cell cycle regulatory proteins in T lymphocyte subsets in patients with asthma attack

Abstract

Objective The aim of this study was to observe the effect of pulmonary surfactant (PS)on the cell cycle distribution and expression of cell cycle regulatory proteins in T lymphocyte subsets in patients with asthma attack and evaluate the molecular regulatory mechanism of PS on T lymphocyte subsets cell proliferation cycle during asthma attack and its therapy significance. Methods The CD4+、CD8+ T lymphocytes obtained from peripheral blood of 30 patients with asthma attack were incubated with PS,the cell cycle and the expression of p27kipl , CyclinE, CyclinA, CyclinB in CD4+ , CD8+ T lymphocytes with PS and without PS were anasyzed by flow cytometry. Results CD4 + T lymphocytes with PS progressed into S phase and G2/M phase were significantly lower than those without PS( P ＜0.01). But remained in G0/G1 phase were significantly higher than those without PS (P ＜ 0.01). CD8+ T lymphocytes with PS progressed into S phase were significantly lower than those without PS( P ＜0.01),G2/M phase were little lower than those without PS( P ＞ 0.05), Go/G1 phase were higher than those without PS( P ＜0.05). The expression of p27kipl in CD4+T lymphocytes with PS was higher than that without PS( P ＜0.05). The expression of cyclinE in CD4+ T lymphocytes with PS was significantly lower than that without PS( P ＜0.01 ). The expression of cyclinA,cyclinB in CD4+ T lymphocytes with PS were lower than those without PS( P ＜0.05). The expression of p27kipl in CD8+ T lymphocytes with PS was higher than that without PS( P ＜0.05). The expression of cyclinE and cyclinA in CD8+ T lymphocytes with PS were significantly lower than that without PS( P ＜0. 01). The expression of cyclinB in CD8+ T lymphocytes with PS was little lower than that without PS( P ＞0.05). Conclusions During asthma attack,PS can inhibit the DNA synthesis, cell division and proliferation of CD4+T lymphocytes by inhibiting positive cell cycle regulatory proteins cyclinE, cyclinA, cyclinB expression and increasing negative cell cycle regulatory protein p27kipl expression in CD4+ T lymphocytes. PS can inhibit the DNA synthesis of CD8+ T lymphocytes by inhibiting positive cell cycle regulatory proteins cyclinE, cyclinA,expression and increasing negative cell cycle regulatory protein p27kipl expression in CD8+ T lymphocytes. Key words: Asthma; Pulmonary surfaetant; T lymphocyte subsets; Cell cycle; Cell cycle regulatory proteins