Abstract
BACKGROUND AND OBJECTIVE: Acute lymphoblastic leukemia (ALL=Acule Lymphoblastic Leukemia) is the most common type of hematological malignancies in Iranian children. Pterostilbene (PT) is a natural stilbenoid in blueberries. Since the effect of PT on lymphoblastic leukemia cell line has not been investigated, the aim of the present study was to evaluate the effects of PT induction and cellular proliferation inhibition on this cell line. METHODS: In this experimental study, Jurkat cell line was cultured in standard conditions with different concentrations of PT (0, 30, 40, 50, 60, 80 and 100 Dm) for 24, 48 and 72 hours and compared with the cell of control group. Then, the effect of PT on cell viability and induction of apoptosis was measured using MTS assay and Annexin V-FITC kit. FINDINGS: Inhibition of cellular proliferation was 44, 47 and 57 at 60 Dm concentration and 71, 78 and 89 at 100 Dm concentration after 24, 48 and 72 hours of PT incubation, respectively (p<0.05). Cell apoptosis was 60 at 80 Dm, 50 at 60 Dm and 27 at 40 Dm compared to 4.63 in control. There was significant difference at 80 Dm concentration. CONCLUSION: The results demonstrated that the PT was effective on the proliferation inhibition and induction of apoptosis in Jurkat lymphoblastic leukemia cell line. The study of the mechanism of apoptosis induction by using this combination can be a progressive step into target therapy. © 2014 Babol University of Medical Sciences. All rights reserved.
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