Abstract
Recently, it was frequently demonstrated that fertility of sows after artificially inseminated is lower than after mating. This is associated with a reduced fertilization capacity of overdiluted insemination doses. The aim of this study was to investigate the sperm motility in the semen samples, forming from the ejaculates with high or low protein content, stored in vitro on 17oC for 3 days. Progressive motility was significantly higher (p
Highlights
After 3 days of storage, in the1:4 dilution proportion, the average progressive motility was significantly (p
The number of good diluted semen samples (≥65% progressive motility), was significantly (p
Progressive motility of good diluted semen samples, was higher in the samples made from the HPC semen (71%), compared with samples made from the LPC semen (66%) (Table 1). These results clearly demonstrated that seminal plasma protein concentration significant influence the progressive motility, both in the native and diluted semen
Summary
About 99% of worldwide pig artificial insemination (AI) is performed with extended liquid semen, stored at 15 to 20oC for 0 to 5 days, with 85% of AI doses using within the day of collection or on the following day (Kommisurd et al, 2002; Stančić and Dragin, 2011; Kalifa et al, 2014).In the classic intracervical AI,inseminationdosevolume80ml to100mlof extended liquid semen, with 3 109 to6 109motile spermatozoa(mean4 109) are used (Johnson et al, 2000; Khalifa et al, 2014 ), with about 1,200 to 1,500 AI doses per boar per year (Glossop, 1998; Stančić, 2000; Singleton, 2001; Stančić and Dragin, 2011).These number of annually doses production per boar has been more often defined as insufficient in modern industrial pig production (Singleton, 2001; Stančić et al.,2009).This is the reason for the creation of a larger number of AI doses per ejaculate, which requires a higher degree ofejaculate dilution. It has been found that overdilution of seminal plasma, for example in overdiluted AI doses, reduce the sperm progressive motility (Maxwell et al 2007; Caballero et al, 2008; Stančić et al, 2012). It is the result of reduction the protein concentration in seminal plasma (Strzeżek et al, 2005; Garcia et al, 2009). Significant variation of protein content in the seminal plasma between individual boars was found (Flowers, 2001; Novak et al, 2010)
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
