Abstract
ABSTRACTAn experiment was conducted to evaluate effects on growth, body composition and nutrient digestibility of addition protease and phytase to soybean meal-based diet in rainbow trout. Seven soybean meal-based diets were prepared by adding protease, phytase and mixture of both enzymes at two different levels (1 and 2 g kg−1) to a base diet without enzymes. Mixed enzymes (1, 2 g kg−1) were prepared by adding protease and phytase at the same doses (0.5 + 0.5 and 1 + 1 g kg−1). Diets consisted of 31% fish meal and 44% dehulled hexane extracted soybean meal. At the start of the experiment, 45 rainbow trout (initial mean body weight 88 g) were stocked into each of 21 fibre tanks. After 90 days, there were no significant differences on the growth, feed conversion ratio, digestibility of protein and lipid among the groups. Results of this study showed that the addition of protease or phytase as mix to soybean meal-based diet could not increase growth and nutrient digestibility in trout.
Highlights
Total aquaculture production in Turkey at 2015 has reached to 240,000 ton
Seven soybean meal-based diets were prepared by adding protease, phytase and mixture of both enzymes at two different levels (1 and 2 g kg−1) to a base diet without enzymes
After 90 days, there were no significant differences on the growth, feed conversion ratio, digestibility of protein and lipid among the groups
Summary
Total aquaculture production in Turkey at 2015 has reached to 240,000 ton. One hundred and eight thousand tons of this production constitutes trout production (Anonymous 2016). Soybean meal is mainly used in rainbow trout feed produced in Turkey (Yıldırım 2008; Korkut et al 2016). It contains antinutritional factors such as trypsin inhibitors that reduce the digestibility of protein (Cheng & Hardy 2003). There are studies on the addition of different enzymes to different feedstuff in fish diet. There are only a few studies on the addition of protease and phytase individually to soybean meal in fish diet. There is no study on the addition as a combination protease and phytase to feedstuff in fish
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