Effect of protamine on ion conductance of upper portion of descending limb of long-looped nephron from hamsters

Abstract

To estimate the contribution of paracellular shunt pathway to the cation-selective permeability in the upper portion of the descending limb of long-looped nephron (LDLu) of hamsters, we observed the effect of protamine on salt-diffusion voltage (delta VT) and transmural resistance (RT). delta VT generated on reduction of lumen NaCl concentration was decreased from 12.0 +/- 1.4 to 7.3 +/- 1.2 mV when 100 micrograms/ml protamine were added to the lumen. Although the effect of protamine persisted after removal of the agent from the lumen, addition of 30 U/ml heparin reversed the delta VT toward the control level. The effect of protamine was dose dependent in the range from 3 to 1,000 micrograms/ml. Protamine was without effect from the bath. Studies on single salt dilution voltage revealed that 100 and 300 micrograms/ml protamine inhibited relative Na+ to Cl- permeability from 4.03 +/- 0.38 to 2.14 +/- 0.21 and from 3.75 +/- 0.37 to 1.36 +/- 0.09, respectively. Protamine markedly decreased the apparent transference number for Na+ but slightly increased the value for Cl-. Protamine also inhibited permeabilities for K+, Rb+, and Li+ relative to Cl-, indicating that the inhibitory effect of protamine was not confined to Na+ but was generalized to cations. Transmural cable analysis showed that 100 micrograms/ml protamine increased RT from 14.0 +/- 1.1 to 19.3 +/- 1.2 omega.cm2, with the effect being reversed by 30 U/ml heparin. Because the effect of protamine on RT was unaffected by ouabain in the bath, changes in RT may mainly represent those of the paracellular shunt resistance.(ABSTRACT TRUNCATED AT 250 WORDS)