Effect of prostaglandin J_2 nanoparticles on repairing exercise-induced bone defect

Abstract

The objective of this study was to investigate the effect of prostaglandin J_2 (PGJ_2) nanoparticles loaded with recombinant human bone morphogenetic protein 2 (rhBMP-2) for 15 days on repairing exercise-induced bone defects. Protein imprinting and real-time quantitative polymerase chain reaction were used to detect the effects of 15-deoxy-Δ12,14-PGJ_2 (15d-PGJ_2) nanoparticles on inflammatory factors in exercise-induced bone defects in rats. The osteogenic differentiation of rhBMP-2–PGJ_2 nanoparticles was detected by alkaline phosphatase staining and alkaline phosphatase content and osteocalcin content determination. The results show that 15d-PGJ_2 could inhibit the protein and gene expression of interleukin 6, interleukin 1 beta and tumor necrosis factor alpha in the tissues surrounding exercise-induced bone defects and upregulate the gene expression of bone morphogenetic protein 6 and platelet-derived growth factor subunit B, and that rhBMP-2–PGJ_2 nanoparticles could promote alkaline phosphatase staining, alkaline phosphatase activity and osteocalcin content. The following are the conclusions: 15d-PGJ_2 nanoparticles can inhibit the inflammatory reaction and increase the osteogenic activity in the exercise-induced bone defect area, rhBMP-2–PGJ_2 nanoparticles have a good effect on promoting the osteogenic differentiation of cells and both of them have a certain repair effect on the exercise-induced bone defect.