Abstract
The effect of propranolol on prolyl hydroxylase activity in blood vessels of rats was investigated in vitro and in vivo. Propranolol at a concentration of 6.7 × 10 −6 M inhibited 50 per cent of the activity of prolyl hydroxylase in the aorta in vitro. The inhibition was recovered when excessive amounts of ferrous iron and ascorbate were added to the reaction mixture. Propranolol showed a competitive type of inhibition with respect to varying concentrations of substrate. Hypertensive rats were made by treatment with desoxycorticosterone acetate and 1% sodium chloride (DOCA-salt). When propranolol was given daily to hypertensive rats for 4 weeks simultaneously with DOCA-salt, no effect on blood pressure was detected, but the activity of prolyl hydroxylase in the aorta and mesenteric artery was significantly inhibited. Prolyl hydroxylase activity in the blood vessels in normotensive rats treated with propranolol was also inhibited. These results suggest that propranolol inhibits prolyl hydroxylase activity in vitro and in vivo at the site of action where an oxygen intermediate is formed from the interaction of ferrous iron and ascorbate.
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