Abstract

Objective: This study aimed to analyze the effect of propolis honey candy consumption on lactoperoxidase (LPO) activity in stimulated saliva.Methods: Stimulated saliva samples were collected from subjects before and after propolis honey candy consumption twice a day for 7 days, and theLPO activity was measured by optical density using a microplate reader.Results: The LPO activity before and after propolis honey candy consumption was found to be 0.010 and 0.013, respectively.Conclusions: A statistically significant increase in the LPO activity after propolis honey candy consumption was observed (Wilcoxon test; p <0.05).

Highlights

  • Propolis is a sticky, dark brown, mixed resin material that is collected by honey bees from various plants

  • LPO activity was calculated for the average triplo absorbance score of saliva minus the average of blank absorbance

  • This study showed that LPO activity increases after the consumption of honey propolis candy and honey candy (Fig. 1)

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Summary

Introduction

Propolis (beeswax) is a sticky, dark brown, mixed resin material that is collected by honey bees from various plants. Propolis is proven to have various biological activities such as antibacterial, antifungal, antiviral, anti-inflammatory, antitumor, and antioxidant activities [3,4]. Propolis consists of 50% resin, 30% wax, 10% essential and aromatic oils, 5% pollen, and 5% other substances [4]. Flavonoids have antibacterial activity, which inhibits bacterial growth through destruction of cell walls, microsomes, and lysosome permeability, as a result of the interaction between flavonoids and the host DNA. This leads to decreased production of hydrogen peroxide (H2O2) by bacteria. This decreasing amount of H2O2 can inhibit enzyme activities such as increasing lactoperoxidase (LPO) in saliva [5]

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