Abstract
：Objective To investigatethe effect of propofol pretreatment on hippocampal monocyte chemotactic protein-1 ( MCP-1) and CC-chemokine receptor type 2 (CCR2) expression following forebrainischemiarepcrfusion (I/R) in rats. Methods Twenty-four male SD rats weighing 250-300 gwere randomly divided into 3 groups ( n = 8 each): group Ⅰ control; group Ⅱ I/R and group Ⅲ propofol pretreatment. Cerebral I/R was induced by clampingbilateral common carotid arteries for 10 min combined with hypotension ( MAP wasmaintained at 35-45 mm Hg) induced by exsanguinations in group Ⅱ and Ⅲ. In group Ⅲ propofol 50 mg/kg was injectedinto femoral vein immediately before cerebral ischemia. The animals were sacrificed at 6 hof reperfusion. Hippocampal tissue was obtained for detection of MCP-1 mRNA and CCR2 mRNAand their protein expression by RT-PCR and Western blot technique. Results I/Rsignificantly increased the expression of MCP-1 and CCR2 in hippoeampal tissue as comparedwith control group. Propofol pretreatment significantly attenuated cerebral I/R inducedincrease in MCP-1 and CCR2 expression. Conclusion Propofol pretreatment can significantlyinhibit forebrain I/R-induced hippocampal MCP-1 and CCP2 expression. Key words: Propofol; Chemokine CCL2; Receptors, CCR2; Reperfasioninjury; Brain
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